Detection Sulfonamide in Food by Upconversion Fluorescence Nanotechnology
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, a sensitive and stable method for detecting five-membered heterocyclic sulfonamides in foods was established based on up-conversion fluorescent nanoparticles technology. Using upconverting nanoparticles as energy donors and gold nanoparticles as energy acceptors, a fluorescence resonance energy transfer system was constructed, and the free antigen was quantified by the amount of recovered fluorescence to achieve rapid detection of five-membered heterocyclic sulfonamides. The results showed that the upconverting luminescent nanomaterials conjugated with the antibody and the gold nanoparticles coated with the antigen can be well combined with good sensitivity and specificity. The fluorescence quenching of the upconversion fluorescent nanoparticles was linearly correlated with the concentration of the sulfonamide antigens (in the range of 0.08~100 ng/mL) present in the system, the correlation coefficient was 0.99371, enabling quantification of the sulfonamides and the lowest detection limit was 0.08 ng/mL. At the same time, this method has been successfully applied to milk, and can identify drugs containing five-membered heterocyclic sulfonamides, with simple operation and good specificity. The method of constructing a fluorescence resonance energy transfer system based on upconversion fluorescent nanoparticles technology can provide new ideas to detect residues of five-membered heterocyclic sulfonamides in food.
