Rapid Screening and Quantification of 64 Kinds of Illegally Added Compounds in Different Wines by Ultra Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High-Resolution Mass Spectrometry
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Abstract:
In this study, a method for rapid screening and quantitative analysis of 64 kinds of illegally added compounds in alcohol samples by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-HRMS) was established. The samples were heated to remove alcohol, and then methanol was added for ultrasonic extraction. The resulting extract was topped up to a fixed volume, filtered through a microporous membrane, and separated by a ThermoHypersil GOLD C18 column (100 mm×2.1 mm, 1.9 μm; ammonium formate and acetonitrile as the mobile phase for gradientelution), before analysis. Mass spectrometry conditions were: simultaneous scanning of positive and negative ions, and full scan/data-dependent secondary scan (Full MS/dd-MS2) mode. High-throughput screening and quantitative analysis of commercially available alcohol samples were carried out with the database set up based on the retention time of the analyte, primary parentions, and automatically collected secondary fragment ions. The results showed a good linear relationship for each target compound within a certain mass concentration range, with correlation coefficients (r2) >0.99, limits of quantitation (LOQs) ranging from 0.1 to 2.5 mg/kg, recoveries in the range of 88.3%~127.9%, and relative standard deviations as 0.2%~6.2% (n=6). This method is simple, highly selective, rapid, accurate and reliable, there by suitable for rapid screening and verification of 64 kinds of illegally added compounds in alcohol samples.
