Specific Detection of Emetic Bacillus cereus in Foods by Loop Mediated Isothermal Amplification (LAMP) Method
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Abstract:
The loop-mediated isothermal amplification (LAMP) assay for the detection of emetic Bacillus cereus was developed by designing four primers including two inner primers and two outer primers that specifically amplify segments of the cesB gene. Analysis of two emetic B. cereus strains, nineteen B. cereus strains and forty-one non-B. cereus strains demonstrated that this LAMP assay was specific. The detection limit of the LAMP method was 1.49 pg/μL with genomic DNA, 5×103 cfu/mL in pure culture. The detection limit of the LAMP method was 2 cfu/g in arti?cially contaminated rice for either boiling or kit-based DNA isolation method after enrichment at 37 ℃ for 6 h. Among seventy-two food samples, two was identified to be positive by this LAMP method, which was consistent with the international standard method. The result demonstrated that the specific LAMP assay can be used for rapid detection of emetic B. cereus strains in food.
