Exogenous Induction of Ergosterol Synthesis in Agaricus blazei
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Abstract:
To increase the content of ergosterol in Agaricus blazei, the content of ergosterol in hyphae was increased by adding exogenous inducer under liquid culture condition. In this study, methyl jasmonate (MeJA) and salicylic acid (SA) were used as inducers, the concentration, adding time and duration of induction were optimized, and the transcriptional level of genes involving in ergosterol synthesis of A. blazei was analyzed by quantitative real time PCR. As results, the best induction conditions for ergosterol synthesis of A. blazei were adding 70.00 μmol/L of MeJA and 10.00 μmol/L of SA to the culture broth when the hyphal was culture to 8 d and 6 d, respectively, and continuously culturing 2 days under these conditions. The content of ergosterol in the harvested hyphae was respectively 2.35 mg/g and 2.43 mg/g, which increased by 147.37% and 155.79% than control group. Moreover, based on the gene expression profiles involving the ergosterol synthesis of A. blazei, it can be speculated that the induction mechanism of MeJA and SA on ergosterol may be they directly or indirectly initiate the expression of upstream key enzyme genes involving in the ergosterol synthesis, and the synthesis was regulated by the interaction of anabolic-related enzymes participating in metabolism. As a conclusion, the content of ergosterol in mycelia obtained by short time exogenous induction culture has exceeded that in fruiting body, so the mycelia obtained in this study can replace the fruiting body to research and apply the ergosterol of A. blazei.
