Cytotoxicity in Human Colon Adenocarcinoma Caco-2 Cell Induced by Selenite and Selenomethionine
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Abstract:
The cytotoxicity and oxidative stress which was induced by selenite (SeIV) and selenomethionine (SeMet) in Caco-2 cells was investigated.Caco-2 cells were exposed to SeIV, SeMetat the difference dose for 24 h. At the same dose of SeIV and SeMet (0.8 μg Se/mL), the apoptosis of Caco-2 cells was detected by Annexin V-FITC/PI assay. The cell viability was evaluated by MTT assay. The leakage of lactate dehydrogenase (LDH), the activity of the superoxide dismutase (SOD), the glutathione (GSH) content were detected respectively. At the dose of 0.8 μg Se/mL, SeIV increased the apoptosis of Caco-2 cells (p<0.05), but there was no change in SeMet treatment. SeIV (≥0.4 μg Se/mL), SeMet (≥40 μg Se/mL) could all decrease the cell viability in a certain dose range (p<0.05) and as their exposure concentration increased, Caco-2 cell viability was gradually decreased. The IC50 values of Caco-2 cell exposed to SeIV and SeMet for 24 h were determined to be 2.56, 215.55 μg Se/mL, respectively. SeIV≥0.8 μg Se/mL and SeMet≥40 μg Se/mL showed the higher leakage of LDH than the control group 14.80% (p<0.05). SeIV≥4 μg Se/mL and SeMet≥4 μg Se/mL could decreased the SOD activity (56.76±3.64 U/mg prot) (p<0.05). SeIV≥4 μg Se/mL and SeMet (160 μg Se/mL) could decreased the GSH contents at the same time (61.67 μg/mg prot) (p<0.05). A certain concentration of SeIV and SeMet can induce oxidative stress in Caco-2 cells, leading to cytotoxicity and inducing apoptosis.
