A Novel Quantum Dot Immunochromatographic Assay for Quantitative Detection of Enrofloxacin
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Abstract:
Enrofloxacin as a widely used antibiotic has attracted widespread attention due to its high positive rate in actual sampling. In this study, an immunochromatographic assay was developed for rapid and quantitative detection of enrofloxacin using quantum dot labeling and tracking. Quantitative detection of enrofloxacin was achieved through investigating the fluorescent quantum dot nanomaterials combined with active materials, developing reagents for sample pretreatment, and screening and optimizing concentrations to achieve the compatibility of quantum dot materials and food substrates. Through the optimization of the whole kit, the linear range of this detection method was 2~1000 ng/ml, the minimum detection limit was 1.80 ng/ml, and the precision was within 10%, with superior performance in accelerated thermal stability tests (stable for 10 days at 37 ℃). Compared with conventional liquid chromatography methods, this established method is simple and rapid with a good correlation among the detection results within the entire detection period of 20 min (correlation coefficient: 0.98). This research fills up the knowledge gap in quantitative detection of antibiotic residues in foods by combining quantum dots and immunochromatography technique, which allows rapid on-site screening of large-scale raw materials and promote the development of rapid food inspection technology.
