Inhibitory Effect of Quercetin, Rutin and Gallic Acid on Xanthine Oxidase and Kinetic Characteristics
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Abstract:
In order to understand the inhibitory properties of quercetin, rutin, gallic acid and its combination with vitamin C on Xanthine oxidase (XOD), a vitro inhibition model of XOD was established to evaluate the inhibitory effect of these three natural compounds on XOD at different concentrations. Allopurinol was used as the positive control. The inhibitory effects and kinetics of these three natural compounds on XOD at different concentrations were detected. The results showed that all the three substances had the inhibitory effect on XOD with concentration dependence, and the inhibitory effect was greatly related to the structure and their nature. The order of inhibition of XOD was as follow: quercetin > rutin > gallic acid. The IC50 values were (5.51±0.41) μmol/L, (48.66±0.49) μmol/L and >200 μmol/L for the three substances respectively. In this work, the inhibition effect of gallic acid was weak and it was demonstrated that vitamin C could assist gallic acid to participate in the reaction. The inhibition rate increased from 20.17% to 40.96%. It could achieve the dual effect of enhanced inhibition of XOD activity and stability. The types of XOD inhibition by quercetin, rutin, and gallic acid belong to different modes of atypical mixed inhibitors. This work will be of great significance to study the inhibition mechanism and application of these substances.
