Analysis Microbial Community on the Surface of Large Yellow Croaker (Larimichthys crocea) Based on High-throughput Sequencing
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Abstract:
To investigate the effect of theaflavin on the microbial composition on the surface of large yellow croaker (Larimichthys crocea) in low-temperature storage, microbial composition and changes in different samples were obtained by microbial extraction and high-throughput sequencing analysis after soaking the refrigerated and frozen yellow croaker with 0.3 mol/L theaflavin and 0.3 mol/L Vc solution. The results showed that the effective sequence range was 22879~55910, and the operational taxonomic unit (OTU) range was 28~49 in large yellow croaker. The number of OTUs in the frozen large yellow croaker after Vc soaking was the lowest. The Chao1 value, Shannon index, and Simpson index of refrigerated and frozen large yellow croaker treated with theaflavin were significantly changed compared with the blank group, among that Shannon index decreased by 17.80% and 13.79%, Simpson index increased by 50.80% and 71.72% respectively, indicating that theaflavin had a significant effect on the colony diversity and richness of bacteria on the surface of large yellow croaker. The surface colony distribution of the large yellow croaker was changed, the relative abundance of Myroides, Psychrobacter and Flavobacterium on the surface of refrigerated large yellow croaker treated with theaflavin decreased from 20.55%, 11.02% and 7.38% to less than 1%, and the growth of Acinetobacter was inhibited by theaflavin treatment. Taken together, theaflavin had a good antioxidative effect on the microbial composition on the surface of large yellow croaker with high-fat content, which could inhibit bacteria growth and prolong the storage time of large yellow croaker. The research provides a basic reference for the quality assurance of large yellow croaker and the development of compound biological preservative.
