Simultaneous Determination of 12 Active Components in Eucommia ulmoides Oliv. by Variable Wavelength-gradient Elution HPLC
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Abstract:
A variable wavelength-gradient elution HPLC method for simultaneous determination of 12 components in Eucommia ulmoides Oliv. was established. Chromatography conditions were as follows: HiQ Sil C 18 column (250 mm×4.6 mm, 5 μm) was used. Mobile phase was 0.2% formic acid (A)-acetonitrile (B) with gradient elution. Detect wavelength were 208 nm, 277 nm and 240 nm. The result of methodology investigation indicated that this methd was good. Twelve components in Eucommia ulmoides Oliv. from different growth years and parts were determined using this method. This method could be used to determine the 12 components in Eucommia ulmoides Oliv within 60 min. Three wavelengths used in this method could not only maximize the signal of each component, but also stabilize the baseline. Gradient elution could ensure that all 12 components of Eucommia ulmoides Oliv. can be separated completely within 60 minutes, which will provide a reference for the determination of various components in Eucommia ulmoides Oliv. by HPLC. Furthermore, it will provide powerful method and means for the content determination of functional components and quality control of Eucommia ulmoides Oliv.
