Isolation, Identification of Bacillus cereus from Brown Rice and Its Virulence Genes Detection and Antibiotic Susceptibility
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Abstract:
In this study, five strains of bacteria were isolated and purified from one sample of brown rice, which were named BR1, BR2, BR3, BR4 and BR5, respectively. Morphology, biochemical tests, and 16S rRNA combined with gyrB gene sequencing were performed to identify the bacteria. Virulence genes detection and drug sensitivity testing were also performed on the bacteria. The results showed that the biochemical characteristics of the five isolates were consistent with Bacillus cereus, and the strains BR1 and BR4 were identified as B. cereus by sequence analysis of 16S rRNA gene and gyrB gene. The detection results of virulence gene PCR showed that the strains BR1 and BR4 harbor virulence-coding genes hbl, nhe, entFM, bceT and cytK. Drug sensitivity testing showed that the strains BR1 and BR4 were resistant to multiple drugs such as penicillin, ampicillin, cefotaxime, etc., moderately sensitive to amoxicillin, sensitive to multiple drugs such as norfloxacin, ciprofloxacin, gentamicin, etc. This study indicated that a variety of virulence genes in B. cereus brown rice isolates were detected, and the strains were resistant to penicillins, cephalosporins and sulfonamides, which had a potential threat to food safety.
