Inhibitory Effects of Three Kinds of Extracts on α-glucosidase and the Preparation of Compound Soft Capsules
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Abstract:
In order to promote the development of anti-diabetic functional food, this research determined the optimal formula of green tea (Camellia sinensis) extract, kudzu root (Puerariae lobatae Radix) extract and bitter gourd (Momordica charantia L) extract for soft capsules, the optimal processing process, and the stability of soft capsules. Based on the single factor tests, the optimal formula of the three natural extracts was determined by the α-glucosidase inhibitory activity assay, based on the indicators like uniformity, fluidity and sedimentation volume ratio, and the properties of the capsule shell like the color and light transmittance. The stability of the soft capsules was investigated by a stability test. The inhibition of α-glucosidase was the greatest when the ratio of green tea extract, kudzu root extract and bitter gourd extract was 1:3:30, with the IC50 value being 0.92 mg/mL. The compound was prepared according to this ratio. It was found that the content of the prepared compound led to the most desirable suspension, when the three extracts were at the optimal ratio and the ratio of soybean oil: beeswax: soybean phospholipid was 1:1.5:0.025:0.07. The capsule shell was prepared at a ratio of 1:0.45:1 for gelatin, glycerin and water, with the addition of the amaranth red pigment containing 2.00% of glycerin and 0.30% of titanium dioxide. As a result, a red capsule shell with good light-blocking effect and moderate hardness and viscosity. The 6-month accelerated test revealed that the properties, loading difference, and disintegration time of the soft capsules all met the requirements of the Chinese Pharmacopoeia, with the IC50 value for the inhibition of α-glucosidase activity decreased only by 5.33%. In summary, the compound soft capsules containing the three natural extracts had a significant inhibitory effect on α-glucosidase and were stable in nature. The obtained technological parameters can provide a basis for the production of the capsules in the future.
