Extraction Process Optimization and Antioxidant Activity Analysis of Phenolic Acids from Agaricus bitorquis (Quél.) Sacc Mycelium
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Abstract:
The process of ultrasonic-assisted extraction was optimized by single factor experiment and Box-Behnken methods to extract phenolic acid from the mycelium of Agaricus bitorquis (Quél.) Sacc. The crude phenolic acid was isolated by macroporous adsorbent resins, and the antioxidant activity of two kinds phenolic acid was evaluated with five classical antioxidant assessment system. Results showed that the optimal conditions were as follows: ultrasonic power of 130W, ethanol concentration of 90%, extraction temperature of 61 ℃, extracting time of 30 minutes, and liquid/solid ratio of 40: 1(mL/g).The antioxidant activity experiment in vitro showed that the free radical scavenging capacity of DPPH and ABTS in purified phenolic acid (PPA) group were higher than those of Vc group (4.31% and 3.69%) and CPA group (27.45% and 32.45%) (p<0.05), and the half inhibitory concentrations in PPA group were lower than those of Vc group (18.57% and 5.20%) and CPA group (40.78% and 42.52%). The order of antioxidant ability was: PPA>Vc>CPA. The scavenging capacity of hydroxyl radicals and superoxide anion radicals in PPA group were higher than those of the CPA group (8.56% and 30.09%), and the half inhibitory concentrations in PPA group were lower than those of CPA group (17.39% and 53.57%). The order of antioxidant ability was: PPA>CPA. The reducing power of PPA group was comparable to that of Vc group, which was higher than that of CPA group (51.05%). The antioxidant activity of phenolic acid was increased with the increment of the sample concentration. In conclusion, this study revealed that the purified phenolic acid from the mycelium of Agaricus bitorquis (Quél.) Sacc have potent antioxidant effects, it could be used as a good source of natural antioxidant.
