Intervention Effect of Mussel and Oyster Complex on Constipation in Mice with Intestinal Flora Disorder
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Abstract:
In this study, the intervention effect of mussel and oyster compound on antibiotic-induced constipation in mice was investigated. C57BL/6 female mice were administered via gavage with the mixed antibiotic solution, to establish a mouse constipation model with intestinal flora disorder (ME). The intervention group (IC) was intragastrically administered via gavage with mussel and oyster compound (0.02 mL/g·bw). Combined with the 16s RNA gene sequencing technology, the indices including the small intestinal propulsion rate, defecation volume, feed consumption, intestinal flora structure were measured after 7 days to evaluate the mouse constipation model, and the intervention effect of mussel and oyster compound. Compared with the blank group, the small intestine propulsion rate of the ME group decreased by 22.89% (p<0.05), the defecation amount decreased by 41.00% (p<0.05), the average daily feed consumption decreased by 2.68 g/d (p<0.05), and the water content of feces decreased by 19.40% (p<0.05). In the meantime, the diversity of beneficial bacteria in the intestinal flora decreased in ME group, which was manifested in the decrease in abundance of beneficial bacteria such as Prevotella-9, Lachnospiraceae, Enterobacter and Verrucomicrobia. After the intervention with mussel and oyster compound, the IC group had an increased (by 70.00%; p<0.05) small intestine propulsion rate, increased (by 52.00%; p<0.05) defecation volume, increased (by 18.10%; p<0.05) fecal water content, and increased diversity of intestinal flora (as increased abundance of Proteobacteria, Fusobacteria, Akkermansia, Bacteroidetes and Verrucomicrobia), compared with ME group. Also, the composition and structure of the intestinal flora in IC group were similar to those of the blank group. In summary, the mussel and oyster compound could significantly improve the small intestinal peristalsis function of mice with constipation caused by antibiotics, and increase the defecation amount and fecal water content, while regulating the structure and composition of intestinal flora of the mice with constipation and increasing the intestinal beneficial bacteria such as Akkermansia.
