Isolation of Staphylococcus aureus from Pork Source and the Detection of Disinfectant Resistance Genes
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Abstract:
In this work, the pollution of Staphylococcus aureus from pork purchased from the market was investigated. The MIC ranges of quaternary ammonium disinfectant to the isolated strains and the disinfectant resistant genes carrying were detected. The 576 samples of the commercial raw pork in four seasons were collected. The MIC values of the isolated strains were determined by broth dilution methods. The five anti-disinfectant-related genes (qacA/ B, qacC/ D, qacG, qacH, qacJ) were detected by PCR. The 297 strains of S. aureus were isolated from 576 pork source samples, and the total isolation rates was 51.56%. The 158 S. aureus strains were isolated from 374 samples that collected in spring and summer. The 139 S. aureus strains were isolated from 202 samples that collected in autumn and winter. The MIC ranges to benzalkonium bromide were 0.00125~0.01 μg/mL. Among 297 strains of S. aureus, 168 strains were found to carry the disinfectant-resistant genes, and the total detection rate was 56.6%. The positive detection rates for qacA/B, qacC/D, qacG, qacH, and qacJ were 2.36%, 19.86%, 40.74%, 6.73%, 0.67%, respectively. The result showed that the isolation rates from pork source and the resistance genes to disinfectant were relatively high. The S. aureus isolated from pork were sensitive to the quaternary salt-based disinfectant. This study will provides reference for the prevention and control of S. aureus pork isolates.
