Changes of Microflora of Penaeus vannamei Infected by Mycotoxins
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In order to find out the characteristics of microbial phase structure of Penaeus vannamei which was polluted by T-2 toxin, aflatoxin B1, ochratoxin A and vomit toxin in the process of culture, the method of determining the total number of colonies in the poisoned Penaeus vannamei was adopted based on the national food safety standard (GB 47892-2010), and the total number of colonies in the poisoned Penaeus vannamei was calculated, and then isolated, screened and identified from the poisoned Penaeus vannamei The number, species and change trend of microorganisms in different genera were compared with those in non poisoned prawn. The results showed that the microbial species of infected prawn muscle were kept at 2~4 and 4~7 in the first 8 days and 16-20 days of storage,respectively, and the total number of bacteria decreased first, then increased and then decreased. Seven strains of bacteria were isolated from the infected and uninfected prawns. They were identified by 16S rDNA sequencing as Corynebacterium, Chrysobacterium, Shiwanella, Campylobacter, Pseudomonas, Micrococcus and Microbacteria. The matching rate of each strain was over 97%, among them, the strains of chrysobacterium and shiwanella, which had strong corrupting ability to aquatic products, were more than 97% Bacteria and Pseudomonas were detected in the infected prawn, while only Schwarzschild was detected in the blank group. Mycotoxins change prawn muscle, resulting in the decline of prawn muscle quality, thus forming a growth environment suitable for bacteria with strong corruption ability, and generating new strains.
