Screening of Cholesterol-Lowering Lactic Acid Bacteria and the Effects of Prebiotics on Their Growth
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Abstract:
In this study, a highly effective cholesterol-lowering strain was isolated from traditional naturally fermented drinks with high bile salt and acid tolerance. In the acid environment with a pH of 3, the acid resistance rate was 71.53%. The strain could tolerate bile salt for a long time with the tolerance rate of 42.09% after exposure for 16 h. With xylene as the adsorbent, the hydrophobicity of the strain was 35.91%, indicating good adhesion characteristics. The prebiotics not only promoted the growth of the strain, but also improved significantly the cholesterol-lowering ability. Among the four prebiotics, lactulose promoted the greatest increase of the biomass, and the biomass reached the highest level after 16 h. Lactulose exerted the greatest effect on the acid production, with the pH value decreasing to 3.56 and cholesterol removal rate reaching 41.10%. Inulin was poorly utilized by the strain, indicating that the strain exhibited selective metabolism to different prebiotics. Based on morphological observations, the strain was identified Lactobacillus paracasei, named Lactobacillus paracasei S0940. This study provides a theoretical basis for further study of cholesterol-lowering ability in vivo and the development of lactic acid bacteria-containing functional foods.
