Stepwise Extraction and Characterization of High Methoxyl Pectin from Lemon Peel
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Abstract:
In this study, two high methoxyl pectins (HMP-1 and HMP-2) with different properties were extracted from lemon peel with organic acid using a stepwise method. The effects of extraction conditions on the yield and viscosity of HMP-1 and HMP-2 were systematically studied. The physicochemical properties of HMP-1 and HMP-2 were investigated, and the microstructure was examined by atomic force microscopy (AFM). Under the conditions of material to liquid ratio 1:20, pH 1.7, 70 ℃ and 1.5 h, the yield of HMP-1 was 19.22%, viscosity of 1% HMP-1 solution was 104.5 mPa·s, molecular weight of HMP-1 was 550.4 ku, degree of esterification (DE) of HMP-1 was 75.2%, and HMP-1 gel strength was 218.24 °SAG, with the absolute value of solution potential being relatively high near the isoelectric point of casein. In terms of HMP-2, under the conditions of material to liquid ratio 1:10, pH 1.7, 80 ℃ and 1.5 h, the yield was 9.74%, viscosity of 1% solution was 49.3 mPa·s, molecular weight was 424.7 ku, degree of esterification was 64.3%, and gel strength was 171.12 °SAG . The exainations with AFM showed that HMP-1 exhibited a relatively long linear shape, whilst HMP-2 was relatively dispersed and finely fragmented. In this study, the stepwise extraction of pectins was realized to obtain different types of pectins, which helps the diversification and precise application of pectins.
