Acetylcholinesterase Amperometric Biosensor Based on Screen-printed Carbon Electrodes for the Detection of Aflatoxin B1
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Abstract:
In order to realize rapid and real-time detection of aflatoxin B1, an electrochemical sensor for detecting aflatoxin B1 was constructed using the screen-printed electrode (which is advantageous in low production cost, fast response, good repeatability and small sample amount), and acetylcholinesterase (which inhibits aflatoxin B1). Using graphene, Prussian blue, chitosan and gold nanoparticles as the modification materials for the screen-printed electrodes, the reaction conditions for the sensor were optimized by cyclic voltammetry. The optimal conditions for the detection of aflatoxin B1 were: pH 7.5, enzyme loading 0.25 U, and inhibition time 14 min. Acetylcholinesterase was used as an inhibitor of the aflatoxin B1, and the obtained biosensor exhibited a linear detection range for aflatoxin B1 from 1 to 64 μg/mL (with a correlation coefficient of 0.9948 and detection limit of 0.05 μg/mL). For the detection of peanut samples, the sensor had a spiked recovery rate in the range of 82.5%~114.1%, with good reproducibility, stability and selectivity. The detection limit was lower than that for the aflatoxins in the peanuts in China, and the developed sensor can be used for the detection of aflatoxin B1 in peanuts.
