Comparative Study of Determination Methods of Total Flavonoids in Vine Tea (Ampelopsis grossedentata)
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Abstract:
The accuracy of direct measurement method, Al(NO3)3-NaNO2-NaOH colo rimetric method, AlCl3 colorimetric method and differential spectrophotometry for determining the total flavonoids in vine tea ( Ampelopsis grossedentata) were studied in this work. The accuracy, repeatability, stability, recovery rates of four methods were evaluated. The effects of white granulate sugar, β-CD, CMC-Na, fructose syrup and caramel on the interference for four methods were investigated. The results were validated by high performance liquid chromatography. The result showed that differential spectrophotometry was the most suitable method for determining the total flavonoids in vine tea with dihydromyricetin as the control and without interferent. The RSD for accuracy, repeatability, stability and recovery rates were 0.19%, 2.26%, 1.67% and 100.97%±1.63%, respectively. The addition of interferent with different concentration could cause the obvious interference to the detection results of the four methods. In the presence of complex interferent, the contents of total flavonoids determined by direct measurement method were most close to that determined by high performance liquid chromatography.
