Research on Screening and Detection Technology of Macamides with Mass Fragmentation Patterns and UPLC-Q-TOF
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Abstract:
A qualitative screening library from the mass fragmentation patterns of benzyl and m-methoxybenzyl macamides was constructed based on the theory of charge or free radical center induction by ultra performance liquid chromatography-quadrupole-time of flight(UPLC-Q-TOF), and a quantitative determination method for 4 macamides was also established. The library includes retention time, mass-to-charge ratio and molecular structure of the excimer ions and fragment ions, and other information. The results showed that not only ten macamides were completely separated, but also more than 10 fragment ions for each macamide could be matched in library with a mass analysis error smaller than 10 Mu. The peak times of the macaamides (A-J) were 5.83, 5.32, 5.93, 6.50, 6.40, 5.23, 7.32, 6.58, 6.47, 7.32 min, respectively. In quantitative detection, the detection limits of 4 macamides (Mb18:3, Mo18:2, Mb18:2, Mb16) were 0.50, 1.20, 1.20, 0.50 mg/kg, respectively, and the quantitative limits were 1.50, 3.60, 3.60, 1.50 mg/kg, respectively. An average recovery rate ranged from 81.6% to 113.0% (RSD<10%) for 4 macamides. This method had high accuracy and precision, puting great significance for origin traceability, identification, quality classification and market supervision of maca and its products.
