Apoptotic Effect of Sophora Flavescens Polysaccharide on Human Lung Cancer A549 Cells
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Abstract:
To explore the effects of Sophora flavescens polysaccharide on proliferation, apoptosis, invasion and migration of human lung cancer cell lines, human lung cancer A549 cell lines were cultured and divided into blank group, drug control group and low, medium and high concentration groups. Cell proliferation, apoptosis, invasion and migration ability were detected in each group, and the expressions of apoptosis-related proteins Bcl-2, PI3K and Akt were determined. The results showed that the cell proliferation rate and apoptosis rate of the high concentration group, at 72 h, were 18.65% and 79.55% respectively, which were superior to those of the other control groups. The number of cell invasion and migration in high concentration group was lower than that in other groups (p<0.05). The relative expressions of Bcl-2, PI3K and Akt in high concentration group were 0.32, 0.40 and 0.34 respectively, which were lower than those in other groups (p<0.05). Under the intervention of Sophora flavescens polysaccharide, the proliferation rate of lung cancer cells decreased, the apoptosis rate increased, the invasion and migration ability were inhibited obviously, and the expression of apoptosis-related proteins Bcl-2, PI3K and Akt were obviously regulated, and their ability showed obvious concentration dependence. This indicated that Sophora flavescens polysaccharide could inhibit the proliferation, invasion and migration of lung cancer cells, promote apoptosis of lung cancer cells and provide a theoretical basis for clinical treatment of lung cancer.
