Effect of Water Extract of Rhizoma Arisaema on the Expression of PKM2 and mTOR Genes in Gastric Cancer Rat Cells
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Abstract:
To investigate the effect of water extract of Rhizoma Arisaema on the expression of PKM2 and mTOR in gastric cancer rat cells. 50 SD male rats were selected, 10 of which were used as the normal group, and the remaining 40 were used to establish the gastric cancer model. The rats were divided into gastric cancer group, drug control group, low and high concentration group, and were treated with intervention. The proliferation and apoptosis of cancer cells in gastric cancer rats were observed, and the expressions of motilin, gastrin, PKM2, mTOR, bcl-2, PI3K and Akt in each group were detected. The results showed that the levels of motilin and gastrin in the high-concentration group were 137.65 pg/ml and 88.76 pg/ml, respectively, and the apoptosis rate was 49.73%, which were all higher than those in the gastric cancer group, drug control group and low-concentration group (p<0.05). The proliferation rate of rats in the high-concentration group was 7.55%, and the relative expression levels of PKM2, mTOR, bcl-2, PI3K and Akt were 1.37, 1.35, 1.37, 1.42 and 1.39, respectively, which were lower than those in the gastric cancer group, drug control group and low-concentration group (p<0.05). The results showed that the gastric function of gastric cancer rats was improved, the proliferation rate of cancer cells was decreased and the apoptosis rate was increased. The expression of PKM2, mTOR, Bcl-2, PI3K and Akt was obviously regulated by the water extract of rhizoma Arisaema. The anticancer ability of the water extract of Rhizoma Arisaema was concentration-dependent, indicating that the water extract of Rhizoma Arisaema could inhibit the proliferation of gastric cancer cells, promote apoptosis of gastric cancer cells and provide a theoretical basis for clinical treatment of gastric cancer.
