Extraction of Total Flavonoids from Toona sinensis Old Leaves by Complex Enzymolysis Assisted with Ultrasound
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Abstract:
In this wok, the extraction method of total flavonoids from old leaves of Toona sinensis was studied. Firstly, cellulose and pectin in the old leaves of Toona sinensis were hydrolyzed by complex enzymes, and the optimal enzymatic hydrolysis condition was found to be complex enzyme 2 (pectinase: cellulase=2:1), with an enzyme dosage of 2.00% (W/V), enzymatic hydrolysis time of 40 min, enzymatic hydrolysis temperature of 45 ℃, and the total flavonoids yield in the supernatant was 2.49%. Ultrasonic assisted extraction was performed later. The single factor experiment and the Box Behnken were used to optimize the ethanol volume ratio, ultrasonic power, ultrasonic time, ultrasonic temperature. The variance analysis showed that the yield of total flavonoids from old leaves of Toona sinensis was affected by following: the ultrasonic power > ethanol concentration > extraction temperature > extracting time. According to the regression equation, the best extraction conditions were predicted. Under the conditions of ethanol volume fraction of 60%, ultrasonic power of 227 W, extraction temperature of 53.00 ℃ and extraction time of 51 min, the total flavonoids yield of old leaves of Toona sinensis was 3.85%, which was only 0.03% different from the predicted value, indicating that the process parameters were accurate and reliable.
