Extract of Chaenomeles speciosa (Sweet) Nakai Prevents Intestinal Mucosal Injury Induced by Non-steroidal Anti-inflammatory Drugs (NSAIDs) in Mice
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Abstract:
The preventive effect of the extract of Chaenomeles speciosa (Sweet) Nakai (CS) on nonsteroidal anti-inflammatory drug (NSAID)-induced intestinal injuries in mice were investigated, mice were given the CS by intragastric administration for 6 days, and then the diclofenac sodium (5 mg/mL) was given to the stomach for 2 consecutive days on the 5th day to intestinal mucosal lesions were observed Expression levels of glucose transporter protein 78 (GRP-78), tumor necrosis factorα (TNF-α), Toll receptor 4 (TLR4), chop and NLRP were detected. The results of FITC-DT showed that the relative fluorescence value (RFU) of the normal group was 682.7±105.4, with 2008.3±496.1, 1097.8±501.1 and 737.5±275.5 for the model group , low CS dose group, and high CS dose group, respectively. Compared with the normal group, the extent of the small intestinal mucosal injury and permeability for the model group significantly increased. Compared with the model group, different CS doses could significantly reduce the small intestinal mucosal injury in mice, with the mechanism associated with the regulation of the endoplasmic reticulum stress (GRP78 and CHOP) and reduction of intestinal inflammation (TLR4 and TNF-α) by CS. These findings suggest that CS can prevent the damage of the small intestinal mucosa caused by NSAID in mice through regulating the TLR4-endoplasmic reticulum stress.
