Protective Effect of Theacrine on Cadium-induced Mice Kidney Damage
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Abstract:
The degree of cadmium damage to kidney in mice and the protective effect of theacrine on kidney under different doses were studied. In the research, the model of oxidative damage of kidney tissue in mice was established by intraperitoneal injection of cadmium chloride. Fifty male Kunming mice were randomly divided into blank group, injury group and low, medium and high dose protection group, with 10 mice in each group. The blank group and model group were given normal saline of equal volume by gastric perfusion, in the protective group were given theacrine suspension of 10 mg/kg, 20 mg/kg, 40 mg/kg by gastric perfusion. After 14 days of treatment, cervical dislocation was performed and kidney tissues were taken out. The contents of lipid peroxidation (MDA), protein carbonylation (PCO), nitric oxide (NO), catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) in the kidney were detected. The pathological changes of nephropathy were observed. The results showed that compared with the blank group, the contents of MDA, PCO and NO in the kidney tissue of the model group increased significantly (p<0.01), which were 1.89, 3.32 and 1.64 times content of the blank group respectively, while the activities of SOD, CAT and GSH decreased significantly (p<0.01), only 76.25%, 77.12% and 45.61% of the blank group respectively. Compared with the model group, the protective group reduced the contents of MDA, PCO and NO, increased SOD, CAT and GSH activities, and reduced the degree of kidney injury. Pathological sections showed that middle dose of theacrine had the best protective effect on kidney injury. The results showed that theacrine had protective effect on cadmium-induced renal injury in mice. It can effectively reduce the degree of oxidative damage and maintain the balance between enzymes and antioxidant enzymes. The mechanism may be related to antioxidant activity.
