Determination and Analysis of Different Morphology of Formaldehyde in Letinous edodes
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Abstract:
In order to one-time and rapidly determine the dissociative formaldehyde and conjugate formaldehyde in Letinous edodes, the method of combining full automatic nitrogen analyzer and high performance liquid chromatography (HPLC) was established. According to the precipitation conditions with different morphology of formaldehyde in Letinous edodes, the 2 steps were set to distill with the automatic nitrogen analyzer. The first step was distilled dissociative formaldehyde and the second step was adding-acid to distill the conjugate formaldehyde. The conditions of experiment were that distilling-time 4 min, 10% phosphoric acid 40 mL, water-bathing temperature 60 ℃, and water-bathing time 60 min. When formaldehyde concentration is 0.0~10.0 mg/L, the standard curve has good linear relationship, the correlation coefficient r is 0.9995. The recoveries of dissociative formaldehyde were 87.8%~105.9%, the relative standard deviation (RSD) was 3.5%~7.0%; the recovery of conjugate formaldehyde was 89.5%~105.8%, and RSD was 2.5%~6.7%. The automatic nitrogen analyzer could improve the extraction efficiency of different morphology of formaldehyde in Letinous edodes. The content of conjugate formaldehyde in Lentinus edodes accounted for 62.6%~71.8%. The contents of dissociative formaldehyde and conjugate formaldehyde in the umbrella part were higher than that in the stalk part. The method combined with high performance liquid chromatography and the automatic nitrogen analyzer was suitable for the batch detection of formaldehyde in Letinous edodes.
