Separation and Purification of Polyphenols in Moringa Seeds
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Abstract:
Moringa seed polyphenols have been proven to possess excellent antioxidant properties. In order to study the bioactivity and physicochemical properties of Moringa seed polyphenols, it is necessary to further separate and remove impurities from the crude extract of Moringa seeds. In this study, the crude extract obtained via ultrasonic-assisted extraction was subjected to further purification. The adsorption rate and desorption rate of the polyphenols from Moringa seeds on different types of macroporous resins were determined, to screen the most suitable macroporous resin for separating and purifying polyphenols. The optimal conditions for the separation and purification of Moringa seed polyphenols were studied by static and dynamic adsorption and desorption experiments. The results showed that D-101 macroporous resin performed the best (which was used to determine the optimal adsorption and desorption conditions). The optimal conditions were: pH and concentration of the sample solution, 5 and 3 mg/mL; Eluent, 80% ethanol solution; olumn flow rate, 2 mL/min; Elution flow rate, 2 mL/min. The purity of the Moringa seed polyphenol extract increased from 10.37% to 32.29%, after the extract was treated under such conditions. The purification effect was significant, thus the findings of this study provided guidance for the separation and purification of Moringa seed polyphenols.
