Antibacterial Activity and Mechanism of Eugenol against Multidrug-resistant Escherichia coli
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Abstract:
In order to study the antibacterial activity of eugenol on multidrug-resistant Escherichia coli (MDR E.coli), the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of eugenol against MDR E.coli were determined by micro-diluted dilution method and agar diffusion method. UV spectrophotometry and the Coomassie blue method were used to analyze the antibacterial activity. The results showed that the MIC and MBC of eugenol for MDR E.coli were 2.50 mg/mL and 5.00 mg/mL, respectively. Eugenol can prolong the progression of MDR E.coli into the logarithmic growth phase, change the structure of the bacteria and increase the soluble protein content and the amount of extracellular nucleic acids of the strain. Eugenol also increased the conductivity in the culture medium. These may be attributed to the destruction effect of eugenol on cellular structure of the bacteria. The study provides a new way to overcome drug resistance, infection and mortality of MDR E.coli, and lay a theoretical foundation for the application of eugenol in medicine and food development.
