Inhibitory Effect of Artemisia argyi Extracts on Xanthine Oxidase Activities and Their Uric Acid-lowering Effect on Hyperuricemia in Mice
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Abstract:
The total flavonoids, total phenols, the inhibitory effect on xanthine oxidase (XOD) activities and free radical scavenging capacity of the extracts from Artemisia argyi by distilled water, anhydrous ethanol assisted with ultrasonic and traditional decoction were measured. Alcohol extract was selected to investigate the effect on hyperuricemia. Potassium oxazidate was used as the inducer for hyperuricemia mice. The mice were continuously fed for 7 days, and serum uric acid (UA), creatinine (Scr), urea nitrogen (BUN) level and liver XOD activity were measured. Results showed that the order of total flavonoids and total phenolics content of Artemisia argyi extracts was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method; the order of free radical scavenging capacity and XOD inhibitory activity was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method.The inhibition mechanism of the three extracts showed irreversible inhibition. The inhibition type of traditional and alcohol extracts effect on XOD is anti-competitive inhibition. The water extract effect on XOD is mixed inhibition. The type of associated inhibition with allopurinol of traditional and water extracts is additive effect. The alcohol extract is weak synergies. The level of UA, Scr and BUN in hyperuricemia mice decreased and the XOD activity of liver was inhibited. The Artemisia argyi extracts have better potential for XOD inhibitory effect and antioxidation. The alcohol extract may be developed to prevent and treat hyperuricemia.
