Preprotective Effect of Actinidia arguta Proanthocyanidins on the Cell Injuried by Drogen Peroxide
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Abstract:
The protective effect of Actinidia arguta Proanthocyanidinss on the Human immortalized epidermal cells (HaCat) injuried by drogen peroxide was investigated. Actinidia arguta in Dawei Mountain of Liuyang was used as test material and HaCat cells cultured in vitro were used as the experimental objects. The experiment was divided into three groups: control group, hydrogen peroxide injury group and treatment group (pretreatment with 5~1000 μg/mL proanthocyanidins of Actinidia arguta), the cell survival rate (apoptosis rate), SOD (superoxide dismutase), GSH-PX (glutathione peroxidase), ROS (reactive oxygen species), Nrf2 gene and protein expression level were measured to evaluate their antioxidant activity. Results showed that when the proanthocyanidins were added to the cells to protect the cells, the survival rate of the injured cells was about 77% at the concentration of 5~500 μg/L proanthocyanidins, which was 2.88 times higher than that in the model group. The levels of SOD (10.46 U/mg prot) and GSH-PX (33.83 U/mg prot) in the treatment group were 108.28% and 137.48% higher than those in the model group (p<0.05), respectively. The level of ROS decreased significantly, and the average intracellular fluorescence intensity decreased by 48.60% compared with the model group (96.21). Meanwhile, the expression of Nrf2 and its protein were 1.40 and 35.11, respectively, which were 63.93% and 80.70% higher than that in the model group. These indicated that procyanidins in Actinidia chinensis have a preprotective effect on oxidative damage of HaCat cells induced by H2O2, and can be further developed into natural and effective antioxidant products to be used in the industry.
