Optimization of Macroporous Resin Purification of Chenodeoxycholic acid and Preliminary Study on Its Lipid Lowering Function
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Abstract:
The purification process of deoxycholic acid and the hypolipidemic function of deoxycholic acid were studied in this paper. Single factor method was used to optimize the separation and purification of deoxycholic acid from chicken bile by macroporous resin. The changes of serum total cholesterol(TC), triglyceride(TG) and high density lipoprotein cholesterol (LDL-C) in hyperlipidemic rats were measured after the administration of the optimized goose deoxycholic acid to the hyperlipidemia rats after 28d. The results showed that D101 macroporous resin had better adsorption and elution effect on goose deoxycholic acid. The best purification conditions were 10 mg/mL, 2 BV/h, 7 BV55% ethanol elution and 5 BV75% ethanol elution. The purity of deoxycholic acid was 98% after elution by D101 macroporous resin. The contents of serum TC, TG and LDL-C were significantly reduced(p<0.05) in the middle and high dose groups of the hyperlipidemic rats fed with goose deoxycholic acid. The results showed that the purification process is simple, environmental pollution is free, the pure goose deoxycholic acid can be obtained, and the pharmacological experiment proved that goose deoxycholic acid has a certain effect on lowering blood lipid.
