Evaluation on the Antioxidant and Anti-inflammatory Activities of the Ethanol Extracts from Urtica Angustifolia in Vitro
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Abstract:
Urtica Angustifolia was used as raw material to evaluate its antioxidant and anti-inflammatory activities in this research in order to develop Urtica Angustifolia resources. Based on the single factor tests, the optimum extraction conditions were determined by response surface methodology. The results showed that the optimum extraction conditions were as follows: extraction time 114 min, solid-liquid 1:15, ethanol concentration 64%. Under the optimal conditions, the ethanol extracts yield was (10.00±0.26)%, and in which the content of active substances total phenols, total flavonoids, procyanidins, saponins, alkaloids were (160.77±0.01) mg/g, (366.85±0.05) mg/g, (7.81±0.01) mg/g, (516.76±0.04) mg/g, (2.01±0.02) mg/g respectively. Meanwhile, Vitamin C and diclofenac sodium were used as antioxidant and anti-inflammatory positive control respectively to evaluate the total reducing power, capacities of scavenging DPPH•, scavenging •OH and activities of inhibiting hyaluronidase and albumin denaturation. The reducing power of the Urtica Angustifolia ethanol extract was higher than Vc (p<0.001) and its EC50 was 0.04 mg/mL. The DPPH? and ?OH scavenging capacities were higher than Vc, and the IC50 on DPPH• and •OH scavenging were 0.02 mg/mL, 1.87 mg/mL respectively. The inhibition of the Urtica Angustifolia ethanol extracts on hyaluronidase and inhibited albumin denaturation activity were similar to diclofenac sodium, and their IC50 values were 2.43 mg/mL and 0.31 mg/mL respectively.