The Regulation of Lipid Metabolism in Rats with Hyperlipidemia by Salvianic acid via Enhanced Expression of LCAT and CYP7A1
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Abstract:
To explore the regulation effect and the mechanism of salvianic acid on the rats with hyperlipidemia. 40 SD rats were randomly divided into normal control group(S, n = 10), hyperlipidemia model group (M, n = 10), salvianic acid high dose group(SAH, n=10) and low dose group(SAL, n =10). Except the control group, the model groupand drug groups were given the high-fat feed to exhibit the hyperlipidemia model. After eight weeks, the blood samples were collected from the abdominal aortic to determinate the serum lipid index in each groupsuch as total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apoAI and apoB by ELISA. HE staining was employed for the detection of liver tissue pathology morphology, RT-PCR and Western-blot technology were also used to detect the mRNA and protein expressions of the lecithin cholesterol acyltransferase (LCAT) and cholesterol 7 alpha hydroxylase (CYP7A1). It was found that salvianic acid could obviously decrease the levels of TC, TG, LDL-C and apoB in the model rats with hyperlipidemia, increase the contents of HDL-C and apoAI, and enhance the expressions of LCAT and CYP7A1(p<0.05). In addition, Salvianic acid could also reduce fat liver lesions, which the effect of high dose groupwas more significant. Thus, salvianic acid could reduce the serum lipid levels in the rats with hyperlipidemia, which its mechanism might be related to the lipid metabolism of the apolipoprotein, LCAT and CYP7A1.
