Effects of Grinding Methods on the Antioxidant and Antiproliferative Activities of Phenolics in Mushroom Caps (Lentinus edodes)
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Abstract:
Three grinding methods were employed on mushroom caps (Lentinus edodes) to generate three kinds of powders, coarse milled cap (CMC), jet milled cap (JMC), and nano-micronized cap (NMC). The polyphenolic content in these three samples and the effect of their antioxidant and antiprofilerative activities on HepG2 cells were determined. The results showed that JMC had the highest amount of free phenolics (4.457±0.076 mg/g) and the lowest amount of bound phenolics (0.274±0.018 mg/g), while CMC had the highest amount of bound phenolics and the lowest amount of free phenolics. The results of the cellular antioxidant activity (CAA) assay showed that the order of CAA values after the three treatments without washing with PBS was: free phenol in CMC (CMC-F) > free phenol in JMC (JMC-F)>free phenol in NMC (NMC-F). The order of CAA values after washing with PBS was: NMC-F > JMC-F > CMC-F. The results of the cell proliferation assay indicated that the free phenolics and bound phenolics after all three treatments had significant effects on inhibiting the proliferation of HepG2 cells. The order of EC50 values was: NMC>CMC>JMC. Therefore, different grinding methods had distinct effects on the polyphenolic content, antioxidant activity, and antiproliferation activity of Lentinus edodes caps. Super?ne grinding could significantly increase the production of total phenolics and free phenolics, decrease the content of bound phenol, and improve the antioxidant activity and antiproliferation activity of Lentinus edodes.
