Internalization Capacity of Salmonella Enteritidis in Mung Bean Sprouts using Artificial Simulation and its Disinfection
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Abstract:
The artificial contamination method was used to study internalization and colonization capacities of Salmonella Enteritidis (ATCC13076) in mung bean sprouts during different stages of germination. Disinfection treatments for internalized Salmonella Enteritidis were also developed. During the four stages (0~48 h) in the pre-germination stage of mung beans (the expansion period [G1, 0 h], the budding period [G2, 12 h], early germination [G3, 24 h], and germination [G4, 48 h]), Salmonella Enteritidis was found to possess different internalization and colonization capacities when inoculated at different concentrations (102, 104, 106, and 108 CFU/mL). When inoculated at a concentration of 102 CFU/mL, Salmonella Enteritidis showed the strongest internalization capability in the germination stage and the seedling stage and at the other two stages, the internalization was relatively weak. With inoculation at a concentration of 104 CFU/mL, Salmonella Enteritidis showed the strongest internalization capacity in the imbibition stage, and with inoculation at a concentration of 108 CFU/mL, the maximum amount of internalized Salmonella Enteritidis in the imbibition stage increased to 2.6×108 CFU/g. Disinfection by ultraviolet radiation can effectively eliminate internalized Salmonella Enteritidis, whereas dip-treatment in sodium hypochlorite solution and silver nitrate solution had no obvious effect.
