Effects of Total Ginsenosides on Expression of CYP2B6 mRNA and Protein in HepG2 Cells in Normal and Inflammatory States
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Abstract:
The purpose of this study was to determine the effects of total ginsenosides on the expression of CYP2B6 mRNA and protein in HepG2 cells during normal and inflammatory states. Total ginsenosides were extracted using the Soxhlet extraction method and colorimetric analysis was employed to measure the concentration of total ginsenosides. The expression levels of CYP2B6 mRNA and protein were detected by RT-PCR and western blot, respectively. The results showed that in the normal state, low, medium, and high concentrations of total ginsenosides could induce the expression of CYP2B6 mRNA in HepG2 cells (p<0.05), and medium and high concentrations could induce CYP2B6 protein expression (p<0.05). LPS treatment of HepG2 cells could significantly increase CYP2B6 protein levels (p<0.05) but had no effect on CYP2B6 mRNA. Compared with the normal state, medium and high concentrations of total ginsenosides could induce the expression of CYP2B6 mRNA after LPS treatment (p<0.05). Low and medium concentrations of total ginsenosides could induce expression of CYP2B6 protein, while the high concentration had an inhibitory effect in LPS-treated HepG2 cells (p<0.05). These results suggested that inflammation state can change the mRNA and protein expression of CYP2B6 induced by total ginsenosides. Therefore, drug-drug interaction between Panax ginseng and other drugs during the normal physiological state and LPS infection should be considered.
