Mechanism of Neurotoxicity Injuries Induced by Carbaryl in PC12 Cells
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Abstract:
The present study was planned to evaluate neurotoxicity injuries induced by carbaryl in PC12 cells, which were used as a model for nervous cell system. In this study, PC12 cells were exposed to carbaryl in different concentrations from 0.00 μg/mL to 200.00 μg/mL, and showed significant changes. Cells viability rates decreased from (100.00±0.46)% to (27.86±1.69)% with a increase of carbaryl concentration. Carbaryl treatment resulted in a significant increase in the levels of LDH leakage rate. MDA content also increased from 19.27±0.96 nmol/mg?prot to 112.39±1.59 nmol/mg prot. SOD activity increased from 4.08±0.87 U/mg prot to 17.77±0.43 U/mg prot, and SOD inhibition rate increased from (19.00±1.66)% to (73.00±1.71)%, however, GSH content decreased from 96.15±6.10 μmol/g prot to 22.91±3.98 μmol/g prot. RFI increased from 37.38±11.48 to 45.56±11.96 firstly, then decreased to 17.11±1.50 with the damage of cells. Ach in medium increased by carbaryl treatment. A significantly decrease in integrity of mitochondrial membrane potential was observed by laser scanning confocal microscope. Carbaryl can induce neurotoxicity in PC12 cells.
