Rapid Detection of Clenbuterol and Procaterol in Meat Samples using Internal Extractive Electrospray Ionization Mass Spectrometry
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Abstract:
Rapid detection of trace levels of β-agonists in meat samples plays an important role in the quality and safety control of meat products. In this study, meat samples, including lean pork, fat pork, pig lung, beef, ham sausage, pig heart, smoked pig feet, and bacon were used as representative samples and were directly analyzed using a homemade internal extractive electrospray ionization (iEESI) source coupled with an LTQ mass spectrometer (MS) under the positive ion detection model. Methanol/water (V/V, 35/65) was selected as the extracting agent; no pretreatment was conducted on the samples, and the mass spectra for chemical fingerprints within an m/z range of 50–1000 were obtained. The collision-induced dissociation (CID) experiments were carried out on the target ions suspected to be “β-agonists”. False positive results were avoided by comparing the characteristic fragment ions with the literature, and a high-throughput screening was performed for traces of β-agonists in meat samples. The results demonstrated that iEESI-MS could rapidly detect clenbuterol, procaterol, and other β-agonists in pork samples; the duration of single sample analysis was less than one minute, and the amount of sample consumed was less than 100 mg. This method has the advantages of simple operation and rapid analysis, and provides a new approach for the quality and safety control of meat products.
