Prevalence and Drug Resistant of Staphylococcus Aureus from Swine Farm and Processing Chain
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Abstract:
To investigate the prevalence of Staphylococcus aureus and drug resistance at pig farms and during slaughter and processing, 507 pork and environmental samples were collected from farms, slaughter and processing, and markets. A total of 136 Staphylococcus aureus strains were identified according to the GB.4789.10-2010. All isolates were tested for antimicrobial susceptibility by the disk diffusion and E-test methods. The presence of genes conferring resistances to tetracyclines, macrolides, and lincosamides as well as the mecA and mecC (mecALGA251) genes carried by methicillin-resistant S. aureus (MRSA) strains in the isolates was examined by polymerase chain reaction (PCR). The results showed that the S. aureus infection rate of fresh pork from slaughterhouses was (44.11%, 60/136), and this value differed significantly from the rates at other sources (P < 0.05). The resistance rates to penicillin, tetracycline, and clindamycin, which are used to treat S. aureus infection, were (92.64%, 126/136), (56.62%, 77/136), and (50.00%, 68/136), respectively. The multidrug resistance rate was 56.62% (77/136), and the main resistance profiles of S. aureus strains were CIP/P/CN/TE/CLR/SXT/DA, P/CLR/DA, and CIP/P/TE/CLR/C/SXT/RD/DA. The drug-resistance genes carried by S. aureus stains posed a serious problem, and gene with the highest detection rate was the tetracycline-resistant gene tetK (90.44%). This study provides a theoretical basis for controlling the spread of multi-resistant strains of S. aureus in the food chain.
