Study on the Diversity of Endophytic Bacteria in Maize using Illumina MiSeq High-throughput Sequencing System
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Abstract:
To investigate the diversity of the endophytic bacterial communities, high-throughput sequencing technique was used to measure the sequence of 16S rDNA-V4 variable region of endophytic bacteria in maize. QIIME and Mothur software programs were employed to sort and calculate the number of sequences and operational taxonomic units (OTUs) for each sample. Thereafter, the abundance, distribution, and alpha diversity index of species and the differences of species abundance among the samples were analyzed. The number of effective sequences and OTUs for each sample was 96334/156; the rarefaction curves showed a sufficient sequencing depth, and the number of OTUs was close to saturation. The Chao1 and Shannon indices of sample YM were 156.0 and 1.211, respectively. The endophytic bacteria in maize belonged to the following six genera: Sphingomonas (37.78%), Halomonas (33.33%), Pseudomonas (13.33%), Shewanella (6.67%), Methylobacterium (4.44%), and Pedobacter (4.44%). The dominant species were Sphingomonas and Halomonas, and accounted for 37.78% and 33.33%, of the total bacteria identified, respectively. Illumina MiSeq high-throughput sequencing system provides a more accurate andscientific data resource for the study of endophytic bacteria in plants.
