Effects of Pre-harvest Calcium Treatment on the Storage Characteristics of Hard-end Whangkeumbae Pear Fruits and the Expression of PpEXPA2
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Abstract:
In order to investigate the influence of pre-harvest calcium treatment on the hard-end Whangkeumbae (Pyrus pyrifolia.Nakai.) pear fruits, normal fruits and hard-end fruits were used as test materials. Some of the hard-end pear fruits were sprayed with CaCl2 solutions before harvest, and the fruit storage characteristics, gene expression level of PpEXPA2, and other indicators were determined. The results showed that during storage at 2 ℃, the firmness, adhesiveness, springiness, cohesiveness, and chewiness of the calyx-end area of normal fruit, CaCl2-treated fruit and hard-end fruit showed a downward trend after the initial increase. The firmness and chewiness in both stem end and calyx end areas of CaCl2-treated fruit were lower than those of hard-end fruit. The weight loss rate was highest in normal fruit, followed by in CaCl2-treated fruit. CaCl2 treatment reduced the rot rate of hard-end fruit and inhibited the respiration rate of the fruits. After 180 days of storage, the rot rate and respiration rate of CaCl2-treated fruit were decreased by 18.87% and 59.70%, respectively, compared with hard-end fruit. During the early stage of the postharvest period, the relative expression level of PpEXPA2 in hard-end fruit was significantly higher than those in normal fruit and CaCl2-treated fruit and was then decreased. The relative expression levels of PpEXPA2 in normal fruit and CaCl2-treated fruit gradually increased during the storage period. Pre-harvest CaCl2 treatment may inhibit the gene expression of PpEXPA2.
