Isolation, Identification, Quorum Sensing, and AHL Activity of Aeromonas hydrophila under Different Conditions
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Abstract:
One quorum sensing (QS) strain was isolated from spoilage bacteria of Scophthalmus maximus and by using classical tests and 16S rRNA gene analysis, it was determined to be Aeromonas hydrophila. The changes in the activity of N-acyl-homoserine lactones (AHLs) during the growth stages of the strain and the effect of different culture conditions on the secretion of AHLs were studied by the punch method. The results indicated that the strain Ah-11 could induce Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136 to produce color. The activities of AHLs increased first and then decreased during the growth phase. The different carbon sources influenced the activity of AHLs in the following order: maltose> glucose> sucrose> fructose> lactose> xylose. The ability of Ah-11 to produce AHLs was reduced in weak acid and alkalescent environment; the optimum pH fat which Ah-11 could secrete AHLs was pH 8.0. Higher concentrations of NaCl would not only inhibit the growth of Ah-11 but also inhibit the activity of AHLs. The concentration of NaCl (0.5–1.0 g/100g) had a significant effect on the ability of Ah-11 to secrete AHLs. The optimal temperature at which the Ah-11 strain could produce AHLs was 28°C and relatively higher or lower temperatures could inhibit AHL secretion. Quorum sensing systems of Ah-11 were regulated by both the density of the bacteria and by environmental factors.
