Antibacterial Activity and Stability of Antimicrobial Peptides in Chinese Prickly Ash Seed Proteins
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Abstract:
Colony count method was used to evaluate the stability of antimicrobial peptides from prickly ash seed proteins and their antimicrobial activity. The results revealed that the peptides showed inhibitory effects on Escherichia coli, Salmonella, Bacillus subtilis, and Staphylococcus aureus. The antimicrobial activity of these peptides increased in a dose-dependent manner. After treating at different temperatures and heating time, the antibacterial activity of the peptides showed no significant differences when compared with that of the control (p > 0.05). The peptides retained excellent antibacterial activity even after the treatment at pH values between 2.0 and 12.0, with lowest activity at pH 2.0 (58.13%) and the highest activity at pH 12.0 (79.17%). With increasing concentration of metal ions, the antibacterial activity decreased, increased, and changed slightly after treatment with K+, Ca2+, and Fe3+, respectively. However, the antibacterial activity increased significantly after treatment with 0.1 mol/L K+. The antibacterial activity decreased slightly after treatment with organic solvents, whereas it increased significantly (p < 0.01) when treated separately with Tween 20 and Tween 80, while the increase was not significant after treatment with SDS. Therefore, the antimicrobial peptides had excellent stability against changes in temperature, pH, metal ion content, and organic solvent concentration, while surfactants (Tween 20 and Tween 80) could significantly improve the antibacterial activity.
