Antibacterial and Coaggregation Activities of Pediococcus pentosaceus and Its Putificatiion Effect on Vibrio parahaemolyticus-contaminated Water
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Abstract:
Coaggregation and antibacterial activity of seven P. pentosaceus strains against Vibrio parahaemolyticus ATCC33847 were evaluated. And their cellular morphological characteristics as well as impact on o Vibrio parahaemolyticus ATCC33847 in contaminated water, were explored. The results showed that the inhibitory effect of the seven P. pentosaceus strains tested was 1.1 to 1.3 times that of lactic acid at the same pH, while the antibacterial activity was resistant to catalase, proteinase, and heat treatments. The 2-h coaggregation rate of the seven isolates against V. parahaemolyticus varied between 14.8% and 37.6%, showing a significant inter-strain difference. Among the seven isolates, the coaggregation activity of F28-8 reached 37.6%, which was significantly higher than those of other strains (p < 0.01). The results from scanning electron microscopy (SEM) showed that strain F28-8 had a unique cell surface structure and cell adhesion pattern. P. pentosaceus also played a role in promoting aggregation and reducing numbers of viable V. parahaemolyticus in water. After a suspension of V. parahaemolyticus was treated with strain F28-8 for 60 h, the concentration of culturable cells in the upper suspension layer was lowered by 3.1 Log10 CFU/mL compared to that of the untreated group, which was significantly higher than that of strain H13, which showed low coaggregation activity (2.5 log10 CFU/mL). On the other hand, the concentration of culturable cells in the coaggregates was 1.6 log10 CFU/mL, which was lower than that of the control group and showed no significant change with H13 treatment (p > 0.05).
