Genotoxicity Biotransformation Products of Low-Dose T-2/HT-2 Toxin
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The work was to determine whether low doses of degradation products of T-2 toxin and HT-2 toxin, after Pseudomonas geniculata biotransformation, showed genetic toxicity. The rates of micronucleated, polychromatic erythrocyte formation in bone marrow and sperm deformity, characterized by a swollen head and folded tail, were measured, 7 d after continual intragastric administration of T-2 and HT-2 toxin biotransformation products, at different doses, in mice. The results showed that the micronucleus formation rate in male mice was higher than that of the females, with significant differences (p < 0.05) between each dose-level and the negative control groups. Additionally, the micronucleus formation rate in male mice from the 10/20 and 25/50 ng/mL dose-level groups showed insignificant differences than that of the positive control group (p > 0.05). Sperm deformity rates in mice from the 2.5/5 ng/mL T-2/HT-2 dose group was significantly different (p < 0.05) than that of the negative group. As the dose of T-2/HT-2 toxin increased, the sperm deformity rate in mice increased linearly. Therefore, T-2 and HT-2 toxins were confirmed to still show genetic toxicity after biotransformation by Pseudomonas geniculate, and males were more susceptible than females. The study also provide evidence that there were binding forms of T-2 toxin in the biotransformation products of T-2 and HT-2.
