Enhancing the Immunogenicity of Hapten 3-Amino-5-morpholinomethyl -2-oxazolidone (AMOZ) Using Dendrimer as a Carrier
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Abstract:
3-Amino-5-morpholinomethyl-2-oxazolidone (AMOZ), a metabolite of antibiotic furaltadone, is a potential teratogenic and carcinogenic substance. However, an immunoassay for the direct estimation of AMOZ was so far unavailable due to its low molecular weight and lack of a specific antibody. In this study, polylysine dendrimer (G5) was used for the first time instead of the traditional carrier (bovine serum albumin (BSA)), to conjugate with AMOZ glyoxylate derivative hapten (AMOZA) to generate the immunogen (AMOZA-G5) that would produce AMOZ-specific antibodies. Balb/c mice were immunized, antisera were collected, and examined by competitive indirect enzyme-linked immune sorbent assay (ciELISA). The results revealed that the antiserum from AMOZA-BSA immunization was not specific to free AMOZ, however, that from AMOZA-G5 immunization showed specificity. ciELISA experiment showed an inhibition rate of 35% for 1 μg/mL AMOZ). The results indicated that polylysine dendrimer as a carrier effectively improved the immunogenicity of small-molecule haptens. This study provides a new strategy for the production of specific antibodies against small-molecule haptens.
