Stimulation of Glucose Consumption in 3T3-L1 Adipocytes by Triterpenoids from Cyclocarya paliurus Leaves
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Abstract:
Cyclocarya paliurus is a unique plant native to China, tea made from the leaves of this plant known for its hypoglycaemic effects. To further explore its hypoglycaemic property, total triterpenoids from Cyclocarya paliurus leaves (CPTT) were extracted by an ultrasound-assisted method and successively purified by extraction, macroporous resin adsorption, and decolorization. The total triterpenoid content of CPTT was as high as 86.3%. The content of ursolic acid, oleanolic acid, maslinic acid, corosolic acid, and betulinic acid in CPTT were 19.35%, 17.38%, 7.53%, 4.59%, and 1.42%, respectively. No obvious change in glucose consumption was observed when 3T3-L1 pre-adipocytes were treated with CPTT in the basal condition, but the consumption significantly increased after simultaneous stimulation with 10 nM insulin. For mature adipocytes, CPTT increased glucose consumption significantly in both basal and insulin-stimulated conditions. For dexamethasone-induced insulin-resistant 3T3-L1 adipocytes, CPTT effectively improved insulin sensitivity and enhanced glucose consumption in a dose-dependent manner, with the optimal effect observed at 25 μg/mL. These results suggest that triterpenoids from Cyclocarya paliurus leaves can effectively increase glucose consumption in adipocytes and therefore have a potential application in both the food and pharmaceutical industries.
