Rapid Detection of Enterohemorrhagic Escherichia coli O157 by LAMP Real-time Turbidity Method
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Abstract:
Real-time monitoring of magnesium pyrophosphate produced from the loop-mediated isothermal amplification (LAMP) reaction was achieved by the turbidimeter. Four specific primers were designed according to the sequences of antigen gene rfbE of EHEC O157. Detection was completed at 63 ℃ on the real-time turbidimeter in 1 h. The specificity, sensitivity, stability of the method was evaluated. Results showed that after optimized, the lowest detection limit was 2.8×103 CFU/mL, and 100 times more sensitive than the PCR, and the minimal adding quantity of 2.8×100 CFU/25 g (or mL) could be detected. LAMP real-time turbidity method is rapid, sensitive, high specificity and easy to operate, which is a good pattern for fast screening of foodborne pathogens.
