Comparison of Methods for Rapid Determination of Total Lipid Content in Microalgae
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Abstract:
The rapid, reliable and easy manipulated method for determination of total lipid content in microalgae is crucial important for screening oleaginous strains, evaluation of functional oil from microalgae and microalgal biological research. Using dry powder of three microalgae (Nannochloris sp., Chlorella vulgaris and Pavlova viridis) as feedstock, the difference of four methods (Bligh-Dyer, modified Bligh-Dyer, ASTM standard and direct saponification methods) was systematically compared in terms of determining total lipid content in microalgae. The fatty acid contents and compositions in crude extracts from different methods were further profiled by GC/MS with in suit transesterification method as control. The results showed that the modified Bligh-Dyer and ASTM standard methods had steady extraction outcomes with the highest fatty acid recoveries at 93.65% vs 93.74%, 85.02% vs 85.77%, and 88.62% vs 88.84% in three powders, respectively. Moreover, no significant difference (p>0.05) appeared in total lipid and fatty acid contents and compositions in extracts by the two methods. Obviously, the modified Bligh-Dyer method is a simple and preferred method with less amount demand of sample (0.25 g) and short time-consuming (1.0 h) for the rapid determination of total lipid content in microalgae, which is an alternative of ASTM standard method in laboratories.
