Inhibition Effect of Quercetin on Aflatoxin B1 Production
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Abstract:
Many polyphenol monomers could inhibit aflatoxin B1 production and Quercetin had higher inhibitory activity than other catechin polyphenols.To explore the inhibition effect of quercetin on aflatoxin B1 production, the antioxidant system of Aspergillus flavus was monitored and the toxin genes express after quercetin treatment were analyzed. The results showed that quercetin treatment decreased the ROS and MDA concentration, confirming that this treatment could alleviate the oxidative stress of Aspergillus flavus. RT-PCR result showed that quercetin was able to activate the transcription factor Yap1 in antioxidant system to increase antioxidant enzyme activity, such as POD, COT and SOD activity. This induction may be a key factor for quercetin inhibition on AFB1. Quercetin downregulated the expression of AflRand AflS And AflS could regulate toxin production by combining AflR expression gene, which is possibly the key mechanism of the inhibition effect of quercetin on AFB1. This was also associated with activation of antioxidant systems in easing the fungi oxidative stress in vivo. In the case of AflR downward, the toxin gene expression was inhibited. Hence, quercetin can be employed as an efficient aflatoxin synthesis inhibitor with great application potential.
